What is the most appropriate test to diagnose COVID-19 in an individual presenting with suggestive symptoms?

09 Feb 2021
Respiratory conditions
  • COVID-19
Type of resource
IPCRG COVID-19 and respiratory Q&A

What the research says:

Diagnostic testing for COVID-19 includes:

Clinical Situation Appropriate Test Testing for Sensitivity (to identify disease) Specificity (to identify no disease Clinical sample Time to result
Symptomatic individual Laboratory-based DNA amplification Presence of viral RNA High High Nasopharyngeal/ oropharyngeal swab 15 minutes to >2 days*
  Laboratory-based antigen testing Presence of viral antigens Moderate High Nasopharyngeal/ oropharyngeal swab 15 minutes to >2 days*
  Lateral flow (point-of-care) test Presence of viral antigens Low to moderate** High Nasopharyngeal/ oropharyngeal swab or finger prick blood sample 15–30 minutes

*Dependent on local laboratory capacity

**Dependent on factors such as for how long somebody has been infected

Antibody testing can be used to indicate past infection, but is not an indicator of current infection.

What this means for your clinical practice:

Confirmation of a suspected diagnosis of COVID-19 infection requires a positive laboratory-based DNA- or antigen-based test. Where laboratory testing is not an option, point-of-care testing can be utilised using a lateral flow test although there is a higher risk of a false negative result.